rps9 proteintech Search Results


rps9  (Proteintech)
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Proteintech rps9
<t>RPS9</t> is up-regulated in osteosarcoma tissues and cell lines. (A) (B) The mRNA and protein expression of RPS9 were measured by qRT-PCR and western blotting in osteosarcoma cell lines (MNNG/HOS, MG63, and U2OS) and in a human normal osteoblast cell line (hFOB 1.19). (C) The grey intensity was calculated in osteosarcoma cell lines and osteoblast cell line. β-actin was used as an internal control. (D) (E) The expression of RPS9 was detected in 20 pairs of osteosarcoma tissues and their adjacent non-tumor tissues. The expression of RPS9 was overexpressed in osteosarcoma tissues compared to that in non-tumor tissues. * P < 0.05.
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RPS9 is up-regulated in osteosarcoma tissues and cell lines. (A) (B) The mRNA and protein expression of RPS9 were measured by qRT-PCR and western blotting in osteosarcoma cell lines (MNNG/HOS, MG63, and U2OS) and in a human normal osteoblast cell line (hFOB 1.19). (C) The grey intensity was calculated in osteosarcoma cell lines and osteoblast cell line. β-actin was used as an internal control. (D) (E) The expression of RPS9 was detected in 20 pairs of osteosarcoma tissues and their adjacent non-tumor tissues. The expression of RPS9 was overexpressed in osteosarcoma tissues compared to that in non-tumor tissues. * P < 0.05.

Journal: Journal of Cancer

Article Title: Down-regulation of RPS9 Inhibits Osteosarcoma Cell Growth through Inactivation of MAPK Signaling Pathway

doi: 10.7150/jca.19130

Figure Lengend Snippet: RPS9 is up-regulated in osteosarcoma tissues and cell lines. (A) (B) The mRNA and protein expression of RPS9 were measured by qRT-PCR and western blotting in osteosarcoma cell lines (MNNG/HOS, MG63, and U2OS) and in a human normal osteoblast cell line (hFOB 1.19). (C) The grey intensity was calculated in osteosarcoma cell lines and osteoblast cell line. β-actin was used as an internal control. (D) (E) The expression of RPS9 was detected in 20 pairs of osteosarcoma tissues and their adjacent non-tumor tissues. The expression of RPS9 was overexpressed in osteosarcoma tissues compared to that in non-tumor tissues. * P < 0.05.

Article Snippet: After blocking in 5% non-fat milk, the membranes were incubated with the following primary antibodies: RPS9 (Proteintech, 1:250), SAPK/JNK (total) (Cell Signaling Technology, 1:1000), SAPK/JNK (Thr183/Tyr185) (Cell Signaling Technology, 1:1000), p38 (total) (BBI Life Sciences, 1:500), p38 (Thr180/Tyr182) (SAB Signalway Antibody, 1:500), or β-actin (Sigma-Aldrich, 1:20,000).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Knockdown of RPS9 inhibits osteosarcoma cell growth in vitro. (A)-(F) Levels of mRNA and protein expression were validated after si-RPS9 transfection in MNNG/HOS cells, MG63 cells, and U2OS cells by qRT-PCR and western blotting, respectively. (G)-(I) Cell Counting Kit-8 (CCK-8) assays were performed after siRNA transfection. (J)-(O) Colony formation assays were performed for RPS9-silenced osteosarcoma cells and control cells. Data are representative of results from three independent experiments. * P < 0.05.

Journal: Journal of Cancer

Article Title: Down-regulation of RPS9 Inhibits Osteosarcoma Cell Growth through Inactivation of MAPK Signaling Pathway

doi: 10.7150/jca.19130

Figure Lengend Snippet: Knockdown of RPS9 inhibits osteosarcoma cell growth in vitro. (A)-(F) Levels of mRNA and protein expression were validated after si-RPS9 transfection in MNNG/HOS cells, MG63 cells, and U2OS cells by qRT-PCR and western blotting, respectively. (G)-(I) Cell Counting Kit-8 (CCK-8) assays were performed after siRNA transfection. (J)-(O) Colony formation assays were performed for RPS9-silenced osteosarcoma cells and control cells. Data are representative of results from three independent experiments. * P < 0.05.

Article Snippet: After blocking in 5% non-fat milk, the membranes were incubated with the following primary antibodies: RPS9 (Proteintech, 1:250), SAPK/JNK (total) (Cell Signaling Technology, 1:1000), SAPK/JNK (Thr183/Tyr185) (Cell Signaling Technology, 1:1000), p38 (total) (BBI Life Sciences, 1:500), p38 (Thr180/Tyr182) (SAB Signalway Antibody, 1:500), or β-actin (Sigma-Aldrich, 1:20,000).

Techniques: Knockdown, In Vitro, Expressing, Transfection, Quantitative RT-PCR, Western Blot, Cell Counting, CCK-8 Assay, Control

Knockdown of RPS9 inhibits osteosarcoma cell G1 cell cycle transition. Representative images of the cell cycle assays in MNNG/HOS cells (A) (B), MG63 cells (D) (E), and U2OS cells (G) (H) after transfection with si-NC or si-RPS9. (C) (F) (I) Diagrams showing the results of cell cycle assay in MNNG/HOS cells, MG63 cells, and U2OS cells respectively. * P < 0.05.

Journal: Journal of Cancer

Article Title: Down-regulation of RPS9 Inhibits Osteosarcoma Cell Growth through Inactivation of MAPK Signaling Pathway

doi: 10.7150/jca.19130

Figure Lengend Snippet: Knockdown of RPS9 inhibits osteosarcoma cell G1 cell cycle transition. Representative images of the cell cycle assays in MNNG/HOS cells (A) (B), MG63 cells (D) (E), and U2OS cells (G) (H) after transfection with si-NC or si-RPS9. (C) (F) (I) Diagrams showing the results of cell cycle assay in MNNG/HOS cells, MG63 cells, and U2OS cells respectively. * P < 0.05.

Article Snippet: After blocking in 5% non-fat milk, the membranes were incubated with the following primary antibodies: RPS9 (Proteintech, 1:250), SAPK/JNK (total) (Cell Signaling Technology, 1:1000), SAPK/JNK (Thr183/Tyr185) (Cell Signaling Technology, 1:1000), p38 (total) (BBI Life Sciences, 1:500), p38 (Thr180/Tyr182) (SAB Signalway Antibody, 1:500), or β-actin (Sigma-Aldrich, 1:20,000).

Techniques: Knockdown, Transfection, Cell Cycle Assay

Knockdown of RPS9 inhibits MAPK signaling pathway in osteosarcoma cells. (A) (B) MNNG/HOS and U2OS cell extracts were prepared and analyzed using the intracellular signaling antibody array kit PathScan ® (#7744). Images were captured using Odyssey ® Infrared Imaging System (LI-COR). (C) (D) Diagrams show quantification of results in MNNG/HOS and U2OS cells respectively. (E) (F) (G) Western blotting analysis of MAPK signaling pathway, including SAPK/JNK and p38, in MNNG/HOS cells, MG63 cells, and U2OS cells respectively transfected with si-NC or si-RPS9.

Journal: Journal of Cancer

Article Title: Down-regulation of RPS9 Inhibits Osteosarcoma Cell Growth through Inactivation of MAPK Signaling Pathway

doi: 10.7150/jca.19130

Figure Lengend Snippet: Knockdown of RPS9 inhibits MAPK signaling pathway in osteosarcoma cells. (A) (B) MNNG/HOS and U2OS cell extracts were prepared and analyzed using the intracellular signaling antibody array kit PathScan ® (#7744). Images were captured using Odyssey ® Infrared Imaging System (LI-COR). (C) (D) Diagrams show quantification of results in MNNG/HOS and U2OS cells respectively. (E) (F) (G) Western blotting analysis of MAPK signaling pathway, including SAPK/JNK and p38, in MNNG/HOS cells, MG63 cells, and U2OS cells respectively transfected with si-NC or si-RPS9.

Article Snippet: After blocking in 5% non-fat milk, the membranes were incubated with the following primary antibodies: RPS9 (Proteintech, 1:250), SAPK/JNK (total) (Cell Signaling Technology, 1:1000), SAPK/JNK (Thr183/Tyr185) (Cell Signaling Technology, 1:1000), p38 (total) (BBI Life Sciences, 1:500), p38 (Thr180/Tyr182) (SAB Signalway Antibody, 1:500), or β-actin (Sigma-Aldrich, 1:20,000).

Techniques: Knockdown, Ab Array, Imaging, Western Blot, Transfection

Clinical significance of RPS9 in patients with osteosarcoma. (A) Representative IHC image of RPS9 in non-tumor tissues. (B) Representative IHC image of RPS9 in osteosarcoma tissues. Original magnification: 50×, 200×.

Journal: Journal of Cancer

Article Title: Down-regulation of RPS9 Inhibits Osteosarcoma Cell Growth through Inactivation of MAPK Signaling Pathway

doi: 10.7150/jca.19130

Figure Lengend Snippet: Clinical significance of RPS9 in patients with osteosarcoma. (A) Representative IHC image of RPS9 in non-tumor tissues. (B) Representative IHC image of RPS9 in osteosarcoma tissues. Original magnification: 50×, 200×.

Article Snippet: After blocking in 5% non-fat milk, the membranes were incubated with the following primary antibodies: RPS9 (Proteintech, 1:250), SAPK/JNK (total) (Cell Signaling Technology, 1:1000), SAPK/JNK (Thr183/Tyr185) (Cell Signaling Technology, 1:1000), p38 (total) (BBI Life Sciences, 1:500), p38 (Thr180/Tyr182) (SAB Signalway Antibody, 1:500), or β-actin (Sigma-Aldrich, 1:20,000).

Techniques:

Correlation analyses of RPS9 protein expression in relation to clinicopathologic variables of 50 patients with osteosarcoma

Journal: Journal of Cancer

Article Title: Down-regulation of RPS9 Inhibits Osteosarcoma Cell Growth through Inactivation of MAPK Signaling Pathway

doi: 10.7150/jca.19130

Figure Lengend Snippet: Correlation analyses of RPS9 protein expression in relation to clinicopathologic variables of 50 patients with osteosarcoma

Article Snippet: After blocking in 5% non-fat milk, the membranes were incubated with the following primary antibodies: RPS9 (Proteintech, 1:250), SAPK/JNK (total) (Cell Signaling Technology, 1:1000), SAPK/JNK (Thr183/Tyr185) (Cell Signaling Technology, 1:1000), p38 (total) (BBI Life Sciences, 1:500), p38 (Thr180/Tyr182) (SAB Signalway Antibody, 1:500), or β-actin (Sigma-Aldrich, 1:20,000).

Techniques: Expressing